Mycobacterium tuberculosis is the second leading cause of death due to a single infectious agent behind disease due to HIV/AIDS. This infection results in immune cell recruitment to the lungs which results in a macrophage rich region (called granuloma) and a lymphocyte-rich region (called lymphocytic cuff). These regions differ in their cell density. The aim of this study was to use H&E stained slides to detect the high cell density regions.
1) Background Segmentation
2) Internuclei Distance Computation
3) Detection of Potential Regions
4) Classification of Potential Regions Into Granulomas, Lymphocytic Cuffs
Figure 1: Flow Chart of Developed Framework for Classification and Detection
Figure 2: Tissue Sample with Green Corresponding to Granulomas and Blue Corresponding to Lymphocytic Cuffs